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rabbit polyclonal antibody against human nestin  (Proteintech)


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    Structured Review

    Proteintech rabbit polyclonal antibody against human nestin
    TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and <t>nestin</t> in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.
    Rabbit Polyclonal Antibody Against Human Nestin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 217 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+human+nestin/Nestin+Antibody/pmc07352379-43-22-29
    Average 96 stars, based on 217 article reviews
    rabbit polyclonal antibody against human nestin - by Bioz Stars, 2026-10
    96/100 stars

    Images

    1) Product Images from "Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells"

    Article Title: Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells

    Journal: Journal of Pain Research

    doi: 10.2147/JPR.S255288

    TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and nestin in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.
    Figure Legend Snippet: TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and nestin in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.

    Techniques Used: Expressing, Immunohistochemistry, Immunofluorescence

    Related Articles

    Binding Assay:

    Article Title: Expression of Cannabinoid Type 1 Receptors in Human Odontoblast Cells.
    Article Snippet: In dental pulp, CB1 receptors could play an important role in dental sensory transduction, biomineralization, tissue repair, and so on.. Related studies of CB receptors could provide a new way for drug treatment of pulp diseases in the future.. Introduction: The aim of this study was to investigate the functional expression of cannabinoid type 1 (CB1) receptors in human odontoblasts (HODs) and the possible internal mechanism.

    Incubation:

    Article Title: Expression of Cannabinoid Type 1 Receptors in Human Odontoblast Cells.
    Article Snippet: In dental pulp, CB1 receptors could play an important role in dental sensory transduction, biomineralization, tissue repair, and so on.. Related studies of CB receptors could provide a new way for drug treatment of pulp diseases in the future.. Introduction: The aim of this study was to investigate the functional expression of cannabinoid type 1 (CB1) receptors in human odontoblasts (HODs) and the possible internal mechanism.

    Article Title: &lt;p&gt;Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells&lt;/p&gt;
    Article Snippet: .. Routine processing was performed as previously described.19 Briefly, slices were incubated with the following primary antibodies: mouse polyclonal antibody against human DSPP (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal antibody against human nestin (1:50; Proteintech Group, Chicago, IL, USA), and rabbit polyclonal antibody against human TRPA1 (1:1000; Santa Cruz Biotech) overnight at 4°C. .. Then, the samples were incubated with the appropriate secondary antibody for 30 min at 37°C: goat polyclonal anti-rabbit immunoglobulin G (IgG) Alexa 594 (Thermo Fisher Scientific, Waltham, MA, USA) or goat polyclonal antimouse IgG-fluorescein isothiocyanate (ZSGB-BIO, Beijing, China).

    Article Title: Expression and distribution of three transient receptor potential vanilloid(TRPV) channel proteins in human odontoblast-like cells.
    Article Snippet: expressed in native HODs and (LHOPs).. The result of qRTPCR and WB confirmed that the gene and protein expression of TRPV1, TRPV2, and TRPV3 channels and TRPV1 mRNA are more abundantly expressed than TRPV2 and TRPV3 in HODs (P < 0.05).. Quantitative analysis of IEM images showed that the relative intracellular distributions of these three channels are similar, and TRPV1, TRPV2 and TRPV3 proteins were preferential labeled in human odontoblast processes, mitochondria, and endoplasmic reticulum.

    Article Title: Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells
    Article Snippet: .. Briefly, slices were incubated with the following primary antibodies: mouse polyclonal antibody against human DSPP (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal antibody against human nestin (1:50; Proteintech Group, Chicago, IL, USA), and rabbit polyclonal antibody against human TRPA1 (1:1000; Santa Cruz Biotech) overnight at 4°C. .. Then, the samples were incubated with the appropriate secondary antibody for 30 min at 37°C: goat polyclonal anti-rabbit immunoglobulin G (IgG) Alexa 594 (Thermo Fisher Scientific, Waltham, MA, USA) or goat polyclonal antimouse IgG-fluorescein isothiocyanate (ZSGB-BIO, Beijing, China).



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    Proteintech rabbit polyclonal antibody against human nestin
    TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and <t>nestin</t> in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.
    Rabbit Polyclonal Antibody Against Human Nestin, supplied by Proteintech, used in various techniques. Bioz Stars score: 96/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+human+nestin/Nestin+Antibody/pmc07352379-43-22-29
    Average 96 stars, based on 1 article reviews
    rabbit polyclonal antibody against human nestin - by Bioz Stars, 2026-10
    96/100 stars
      Buy from Supplier

    90
    Spring Bioscience rabbit polyclonal anti-human antibody raised against nestin
    TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and <t>nestin</t> in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.
    Rabbit Polyclonal Anti Human Antibody Raised Against Nestin, supplied by Spring Bioscience, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/rabbit+polyclonal+antibody+against+human+nestin/rabbit+polyclonal+anti+human+antibody+raised+against+nestin/10__1097_slash_01__xej__0000446921__92764__ab-66-57-60
    Average 90 stars, based on 1 article reviews
    rabbit polyclonal anti-human antibody raised against nestin - by Bioz Stars, 2026-10
    90/100 stars
      Buy from Supplier

    Image Search Results


    TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and nestin in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.

    Journal: Journal of Pain Research

    Article Title: Tumor Necrosis Factor-α Regulates the TRPA1 Expression in Human Odontoblast-Like Cells

    doi: 10.2147/JPR.S255288

    Figure Lengend Snippet: TRPA1 expression in HODs and HOD-like cells. ( A and B ) IHC localization of DSPP and nestin in HODs. ( C ) The expression of TRPA1 in cell bodies and cell processes of HODs. The positive expression of DSPP ( D – F ), nestin ( G – I ) and TRPA1 ( J – L ) in HOD-like cells. P dental pulp, D dentin, ODs odontoblast layer. ( A – C: scale bars=50 μm, D – I: scale bars=100 μm, J – L: scale bars=200 μm). Abbreviations: TRPA1, transient receptor potential ankyrin 1; HOD, human odontoblast; IHC, immunohistochemistry; DSPP, dentin sialophosphoprotein; IF, immunofluorescence.

    Article Snippet: Briefly, slices were incubated with the following primary antibodies: mouse polyclonal antibody against human DSPP (1:100; Santa Cruz Biotechnology, Dallas, TX, USA), rabbit polyclonal antibody against human nestin (1:50; Proteintech Group, Chicago, IL, USA), and rabbit polyclonal antibody against human TRPA1 (1:1000; Santa Cruz Biotech) overnight at 4°C.

    Techniques: Expressing, Immunohistochemistry, Immunofluorescence